Assay of rat plasma pyruvate kinase activity with luciferin-luciferase.
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چکیده
منابع مشابه
Assay of rat plasma pyruvate kinase activity with luciferin-luciferase.
An assay method for pyruvate kinase in rat plasma is described. Plasma samples were incubated with ADP and phosphoenolpyruvate in Tris buffer solution. The ATP produced by pyruvate kinase was measured by photocounting after the addition of a commercially available luciferin-luciferase preparation. Interference by ATP or adenylate kinase originally present in the sample was removed by a high deg...
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ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 1988
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.34.607